Marchisio

Research Interests

The research activity of the Cytomorphology Unit is mainly focused on the study of lipid dependent-signal transduction pathways during cell growth and differentiation. These studies led to the identification and the possible role, in both whole cell and nuclear compartment, of specific isoforms of PKC, PI-PLC and PI3K. The significance of inositide related enzymes and their connected signaling were studied during the overcoming of the differentiation blockade of APL-derived tumoral promyelocytes (leukemic blasts, cell lines as HL-60 and NB4) induced by agonists (trans-retinoic acid, As₂O₃). A more recent research interest concerns the role of specific PLC isoforms, both cytoplasmic and nuclear, in promotion and/or progression of breast cancer. The activity allowed to the identification of molecular targets for antineoplastic strategies. The main skills acquired were related to all aspects of proteins study (functional proteomics), as immunochemical, immunocytochemical and immunohistochemical analysis, positive and negative modulation of protein expression, specific enzymatic activity assays, flow cytometry, high-speed cell sorter flow cytometry and quantitative imaging flow cytometer.

Unit Description

The Unit has years of expertise in the use of multicolour flow cytometry (FCM) and in the application of specific molecular biology techniques. The staff from the Unit can apply nine-colour FCM panels to the study of the heterogeneity of blood compartments, as evidenced by the amount of publications, addressing this issue. In the last three years, the Unit worked on the development of new tools to the detection and count of both circulating endothelial cells and extracellular vesicles. On the other hand, the Unit acquired expertise in cell sorting techniques.

Equipment

See Cytometry Facility

1. Extracellular Vesicles in Feto-Maternal Crosstalk and Pregnancy Disorders. Buca D, Bologna G, D'Amico A, Cugini S, Musca F, Febbo M, D'Arcangelo D, Buca D, Simeone P, Liberati M, Vitacolonna E, Miscia S, D'Antonio F, Lanuti P. Int J Mol Sci. 2020 Mar 19;21(6). pii: E2120. doi: 10.3390/ijms21062120. Review.
2. An explorative study identifies miRNA signatures for the diagnosis of non-celiac wheat sensitivity. Clemente E, Efthymakis K, Carletti E, Capone V, Sperduti S, Bologna G, Marchisio M, Di Nicola M, Neri M, Sallese M. PLoS One. 2019 Dec 13;14(12):e0226478. doi: 10.1371/journal.pone.0226478. eCollection 2019.
3. Circulating Cancer Stem Cell-Derived Extracellular Vesicles as a Novel Biomarker for Clinical Outcome Evaluation. Brocco D, Lanuti P, Simeone P, Bologna G, Pieragostino D, Cufaro MC, Graziano V, Peri M, Di Marino P, De Tursi M, Grassadonia A, Rapposelli IG, Pierdomenico L, Ercolino E, Ciccocioppo F, Del Boccio P, Marchisio M, Natoli C, Miscia S, Tinari N. J Oncol. 2019 Nov 18;2019:5879616. doi: 10.1155/2019/5879616. eCollection 2019.
4. Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation. Ciardiello C, Leone A, Lanuti P, Roca MS, Moccia T, Minciacchi VR, Minopoli M, Gigantino V, De Cecio R, Rippa M, Petti L, Capone F, Vitagliano C, Milone MR, Pucci B, Lombardi R, Iannelli F, Di Gennaro E, Bruzzese F, Marchisio M, Carriero MV, Di Vizio D, Budillon A. J Exp Clin Cancer Res. 2019 Jul 18;38(1):317. doi: 10.1186/s13046-019-1317-6.
5. Extracellular Vesicles and Their Potential Use in Monitoring Cancer Progression and Therapy: The Contribution of Proteomics. Cufaro MC, Pieragostino D, Lanuti P, Rossi C, Cicalini I, Federici L, De Laurenzi V, Del Boccio P. J Oncol. 2019 Jun 9;2019:1639854. doi: 10.1155/2019/1639854. eCollection 2019. Review.
6. Proteomics characterization of extracellular vesicles sorted by flow cytometry reveals a disease-specific molecular cross-talk from cerebrospinal fluid and tears in multiple sclerosis. Pieragostino D, Lanuti P, Cicalini I, Cufaro MC, Ciccocioppo F, Ronci M, Simeone P, Onofrj M, van der Pol E, Fontana A, Marchisio M, Del Boccio P. J Proteomics. 2019 Jul 30;204:103403. doi: 10.1016/j.jprot.2019.103403. Epub 2019 Jun 3.
7. Periodontal Ligament Stem Cells: Current Knowledge and Future Perspectives. Trubiani O, Pizzicannella J, Caputi S, Marchisio M, Mazzon E, Paganelli R, Paganelli A, Diomede F. Stem Cells Dev. 2019 Aug 1;28(15):995-1003. doi: 10.1089/scd.2019.0025. Epub 2019 May 20.
8. Platelet-Derived Microparticles From Obese Individuals: Characterization of Number, Size, Proteomics, and Crosstalk With Cancer and Endothelial Cells. Grande R, Dovizio M, Marcone S, Szklanna PB, Bruno A, Ebhardt HA, Cassidy H, Ní Áinle F, Caprodossi A, Lanuti P, Marchisio M, Mingrone G, Maguire PB, Patrignani P. Front Pharmacol. 2019 Jan 22;10:7. doi: 10.3389/fphar.2019.00007. eCollection 2019.
9. Vav1 downmodulates Akt in different breast cancer subtypes: a new promising chance to improve breast cancer outcome. Grassilli S, Brugnoli F, Lattanzio R, Marchisio M, Perracchio L, Piantelli M, Bavelloni A, Capitani S, Bertagnolo V. Mol Oncol. 2018 Jun;12(7):1012-1025. doi: 10.1002/1878-0261.12203. Epub 2018 May 16.
10. A standardized flow cytometry network study for the assessment of circulating endothelial cell physiological ranges. Lanuti P, Simeone P, Rotta G, Almici C, Avvisati G, Azzaro R, Bologna G, Budillon A, Di Cerbo M, Di Gennaro E, Di Martino ML, Diodato A, Doretto P, Ercolino E, Falda A, Gregorj C, Leone A, Losa F, Malara N, Marini M, Mastroroberto P, Mollace V, Morelli M, Muggianu E, Musolino G, Neva A, Pierdomenico L, Pinna S, Piovani G, Roca MS, Russo D, Scotti L, Tirindelli MC, Trunzo V, Venturella R, Vitagliano C, Zullo F, Marchisio M, Miscia S. Sci Rep. 2018 Apr 11;8(1):5823. doi: 10.1038/s41598-018-24234-0.
11. Enhanced release of acid sphingomyelinase-enriched exosomes generates a lipidomics signature in CSF of Multiple Sclerosis patients. Pieragostino D, Cicalini I, Lanuti P, Ercolino E, di Ioia M, Zucchelli M, Zappacosta R, Miscia S, Marchisio M, Sacchetta P, Onofrj M, Del Boccio P. Sci Rep. 2018 Feb 15;8(1):3071. doi: 10.1038/s41598-018-21497-5.
12. Paragangliomas arise through an autonomous vasculo-angio-neurogenic program inhibited by imatinib. Verginelli F, Perconti S, Vespa S, Schiavi F, Prasad SC, Lanuti P, Cama A, Tramontana L, Esposito DL, Guarnieri S, Sheu A, Pantalone MR, Florio R, Morgano A, Rossi C, Bologna G, Marchisio M, D'Argenio A, Taschin E, Visone R, Opocher G, Veronese A, Paties CT, Rajasekhar VK, Söderberg-Nauclér C, Sanna M, Lotti LV, Mariani-Costantini R. Acta Neuropathol. 2018 May;135(5):779-798. doi: 10.1007/s00401-017-1799-2. Epub 2018 Jan 5. Erratum in: Acta Neuropathol. 2018 Feb 1;:
13. Mechanisms of endothelial cell dysfunction in cystic fibrosis. Totani L, Plebani R, Piccoli A, Di Silvestre S, Lanuti P, Recchiuti A, Cianci E, Dell'Elba G, Sacchetti S, Patruno S, Guarnieri S, Mariggiò MA, Mari VC, Anile M, Venuta F, Del Porto P, Moretti P, Prioletta M, Mucilli F, Marchisio M, Pandolfi A, Evangelista V, Romano M. Biochim Biophys Acta Mol Basis Dis. 2017 Dec;1863(12):3243-3253. doi: 10.1016/j.bbadis.2017.08.011. Epub 2017 Aug 25.
14. Liraglutide mitigates TNF-α induced pro-atherogenic changes and microvesicle release in HUVEC from diabetic women. Di Tomo P, Lanuti P, Di Pietro N, Baldassarre MPA, Marchisio M, Pandolfi A, Consoli A, Formoso G. Diabetes Metab Res Rev. 2017 Nov;33(8). doi: 10.1002/dmrr.2925. Epub 2017 Sep 6.
15. Lipoxin A₄ stimulates endothelial miR-126-5p expression and its transfer via microvesicles. Codagnone M, Recchiuti A, Lanuti P, Pierdomenico AM, Cianci E, Patruno S, Mari VC, Simiele F, Di Tomo P, Pandolfi A, Romano M. FASEB J. 2017 May;31(5):1856-1866. doi: 10.1096/fj.201600952R. Epub 2017 Jan 18.
16. Microparticles as new markers of cardiovascular risk in diabetes and beyond. Santilli F, Marchisio M, Lanuti P, Boccatonda A, Miscia S, Davì G. Thromb Haemost. 2016 Aug 1;116(2):220-34. doi: 10.1160/TH16-03-0176. Epub 2016 May 12. Review.
17. Endothelial progenitor cells, defined by the simultaneous surface expression of VEGFR2 and CD133, are not detectable in healthy peripheral and cord blood. Lanuti P, Rotta G, Almici C, Avvisati G, Budillon A, Doretto P, Malara N, Marini M, Neva A, Simeone P, Di Gennaro E, Leone A, Falda A, Tozzoli R, Gregorj C, Di Cerbo M, Trunzo V, Mollace V, Marchisio M, Miscia S. Cytometry A. 2016 Mar;89(3):259-70. doi: 10.1002/cyto.a.22730. Epub 2015 Aug 25.